The targeting vector contains, from 5' to 3', a partial Cartpt sequence spanning exon 1 through intron 2, an FRT3 site within Cartpt intron 2, a partial Cartpt exon 3 sequence up to and including the endogenous stop codon, an internal ribosome entry site 2 (IRES2) sequence (allows translation initiation in the middle of an mRNA sequence), a Cre ...
Linear Hygromycin Marker is a short ds DNA fragment consisting of the hygromycin resistance gene regulated by the SV40 promoter and ending with the SV40 polyadenylation signal. The 2.1-kb marker is compatible with any expression vector (including Tet System vectors) for generating stable transfectants using CalPhos transfection reagent or a ...
Transcription of the autosomal phosphoglycerate kinase gene, Pgk-2, is initiated at the onset of meiosis during spermatogenesis in mammals. However, in the mouse, the 5′ portion of the endogenous Pgk-2 coding sequence undergoes a specific demethylation event that precedes transcriptional activation by 10–12 days.
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The prokaryotic promoter gb2 is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the normally used Tn5 promoter. The promoter of the mouse Phosphoglucokinase gene (PGK) is used as eukaryotic promoter. A synthetic polyadenylation signal terminates the kanamycin/neomycin expression.
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Jan 23, 2007 · Sequence archive. Help. Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. UniRef. Sequence clusters. Proteomes. Protein sets from fully sequenced genomes. Annotation systems. Systems used to automatically annotate proteins with high accuracy: UniRule (Expertly curated rules) ARBA (System generated rules ...
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We then generated two reporter lines of mice by inserting EYFP or ECFP cDNAs into the ROSA26 locus, preceded by a loxP-flanked stop sequence. These strains were tested by crossing them with transgenic strains expressing Cre in a ubiquitous ( β-actin-Cre ) or a cell-specific ( Isl1-Cre and En1-Cre ) pattern.
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Hi All, I want to put my EGFP under the PGK promoter of Puromycin through IRES for mammalian syst... Removing all stop codons from Sequence Record using Biopython Hello Everyone, I have sequences that occasionally have an erronious stop codon.
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Negative inducible promoter pBad is another popular prokaryotic promoter often used for bacterial protein purification. When arabinose is absent, regulatory protein AraC binds O and I1 sites upstream of pBad, blocking transcription. The addition of arabinose causes AraC to bind I1 and I2 sites, allowing transcription to begin.
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Transcription of a mutated pgk promoter. (A) The sequence upstream of the C. trachomatis pgk promoter is shown with the predicted σ 28 promoter in boldface type, and the mapped transcription start sites are in boldface capital letters. For reference,...
In step1, a cassette containing neomycin phosphotransferase coding sequence (Neo, green) that is expressed from a phosphoglycerate kinase-1 (pgk) promoter (light blue) flanked by loxP elements (red) is targeted to a chosen locus, in this case Hnf3 α (yellow), by homologous recombination.
Effectively, CMV is a very bad promoter for a long term expression in stem cells, PGK or EF1 work better. If you want to test different promoters without annoying you to make a lot of clonages, I ...
For this promoter, there is a cluster of 2 transcription start sites,in the interval 3072928 to 3072929, where the highest frequency is at position 3070951. The most upstream position of this cluster is assigned as TSS position in the promoter.
murine PGK promoter was used to drive GFP expression in the HIV-1 and FIV vectors, as we have previously noted that the CMV promoter functions poorly in feline cells transduced with lentiviral vectors (J.L.A., unpublished data). In contrast to the human bone marrow studies, high levels of GFP expression were achieved in feline